collagenase dispase solution (Thermo Fisher)

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Thermo Fisher collagenase dispase solution
Collagenase Dispase Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+dispase+solution/Collagenase/bio_rxiv__64898__2026__01__09__698753-237-6-10
Average 97 stars, based on 1 article reviews

collagenase dispase solution - by Bioz Stars, 2026-11

97/100 stars

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Incubation:

Article Title: Scalable high-fidelity human vascularized cortical assembloids recapitulate neurovascular co-development and cell specialization
Article Snippet: .. Tissue fragments were incubated in a collagenase/dispase solution (3 mg/mL, Thermofisher) for 30 minutes at 37°C. .. Samples were further triturated in Hank’s Balanced Salt Solution (HBSS) buffer (Sigma Aldrich), supplemented with 1% Bovine Serum Albumin (BSA), 0.1% glucose (Sigma Aldrich) containing 0.25 mg/mL DNase I (Roche) for 30 minutes at 37°C.

Article Title: Hybrid Scaffolds Decouple Biochemical & Biophysical Regulation of Cell Phenotype
Article Snippet: .. Single cells for ex vivo culture were isolated from tumours by mincing with a scalpel and incubation in 12 ml collagenase/dispase solution (collagenase type II 100 U/ml, dispase 2.4 U/ml in HBSS, Invitrogen). .. After 80 min at 37 °C with rotation, the sample was washed through a 70 μm nylon mesh with phenol red free RPMI (R7509 Sigma), followed by centrifugation (300g, 5 minutes) to extract the cell pellet.

Article Title: Hybrid Scaffolds Decouple Biochemical & Biophysical Regulation of Cell Phenotype.
Article Snippet: .. Single cells for ex vivo culture were isolated from tumours by mincing with a scalpel and incubation in 12 mL collagenase/dispase solution (collagenase type II 100 U/mL, dispase 2.4 U/mL in HBSS, Invitrogen). .. After 80 min at 37◦C with rotation, the sample was washed through a 70 μm nylon mesh with phenol red free RPMI (R7509 Sigma), followed by centrifugation (300 g, 5 min) to extract the cell pellet.

Article Title: Hybrid Scaffolds Decouple Biochemical & Biophysical Regulation of Cell Phenotype
Article Snippet: .. Single cells for ex vivo culture were isolated from tumours by mincing with a scalpel and incubation in 12 mL collagenase/dispase solution (collagenase type II 100 U/mL, dispase 2.4 U/mL in HBSS, Invitrogen). .. After 80 min at 37°C with rotation, the sample was washed through a 70 μm nylon mesh with phenol red free RPMI (R7509 Sigma), followed by centrifugation (300 g, 5 min) to extract the cell pellet.

Article Title: Unravelling the distinct phenotype and mechanosensitive properties of different tendon cell populations
Article Snippet: .. After extraction, fascicles were placed in a collagenase/dispase solution (2 mg/mL collagenase type II (Gibco, Thermo Fisher) and 1 mg/mL dispase (STEMCELL Technologies)) and incubated at 37° C with 5% CO 2 with agitation (∼80 RPM). ..

Ex Vivo:

Article Title: Hybrid Scaffolds Decouple Biochemical & Biophysical Regulation of Cell Phenotype
Article Snippet: .. Single cells for ex vivo culture were isolated from tumours by mincing with a scalpel and incubation in 12 mL collagenase/dispase solution (collagenase type II 100 U/mL, dispase 2.4 U/mL in HBSS, Invitrogen). .. After 80 min at 37°C with rotation, the sample was washed through a 70 μm nylon mesh with phenol red free RPMI (R7509 Sigma), followed by centrifugation (300 g, 5 min) to extract the cell pellet.

Isolation:

Article Title: Hybrid Scaffolds Decouple Biochemical & Biophysical Regulation of Cell Phenotype
Article Snippet: .. Single cells for ex vivo culture were isolated from tumours by mincing with a scalpel and incubation in 12 mL collagenase/dispase solution (collagenase type II 100 U/mL, dispase 2.4 U/mL in HBSS, Invitrogen). .. After 80 min at 37°C with rotation, the sample was washed through a 70 μm nylon mesh with phenol red free RPMI (R7509 Sigma), followed by centrifugation (300 g, 5 min) to extract the cell pellet.

Gentle:

Article Title: Mechanistic elucidation of human pancreatic acinar development using single-cell transcriptome analysis on a human iPSC differentiation model.
Article Snippet: .. Then, cells were dispersed in a collagenase/dispase solution consisting of 3.5 mg collagenase type II (GIBCO) and 2 mg dispase II (Wako) dissolved in 1 mL collagenase buffer for 15 min at 37 °C and dissociated into single cells by gentle pipetting. .. Staining with TotalSeqTM-A0951 PE-Streptavidin (BioLegend), TotalSeqTM-A0952 PE-Streptavidin (BioLegend), and TotalSeqTM-A0953 PE-Streptavidin (BioLegend) was used to identify cells on days 0, 11, and 25, respectively.

Article Title: Mechanistic elucidation of human pancreatic acinar development using single-cell transcriptome analysis on a human iPSC differentiation model
Article Snippet: .. Then, cells were dispersed in a collagenase/dispase solution consisting of 3.5 mg collagenase type II (GIBCO) and 2 mg dispase II (Wako) dissolved in 1 mL collagenase buffer for 15 min at 37 °C and dissociated into single cells by gentle pipetting. .. Staining with TotalSeqTM-A0951 PE-Streptavidin (BioLegend), TotalSeqTM-A0952 PE-Streptavidin (BioLegend), and TotalSeqTM-A0953 PE-Streptavidin (BioLegend) was used to identify cells on days 0, 11, and 25, respectively.

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